B10 A-line
| Denomination: | 'B10 A-line' |
|---|---|
| Botanical Name: | Brassica juncea |
| Applicant/Holder: |
Agriculture & Agri-Food Canada, Saskatoon Saskatoon Research Centre 107 Science Place Saskatoon, Saskatchewan S7N 0X2 Canada |
| Breeder: |
Bifang Cheng, Agriculture & Agri-Food Canada, Saskatoon, Saskatchewan |
| Agent in Canada: |
Mustard 21 Canada Inc. 510 Blackthorn Crescent Saskatoon, Saskatchewan S7V 1A8 Canada Tel: 306-262-5333 |
| Application Date: | 2024-02-08 |
| Provisional Protection: | 2024-02-08 |
| Application Number: | 24-11597 |
| Grant of Rights Date: | 2026-02-23 |
| Certificate Number: | 7652 |
| Grant of Rights Termination Date: | 2046-02-23 |
Variety Description
Varieties used for comparison: 'Centennial Brown' and 'B12-A line'
Summary: The cotyledon of B10 A-line' is wider than that of B12 A-line'. The leaf of B10 A-line' is wide elliptic in shape while those of the reference varieties are narrow elliptic in shape. At flowering, the plants of B10 A-line' are taller than those of Centennial Brown'. When all branches are raised vertically, the total plant length of B10 A-line' is longer than that of Centennial Brown'. The silique, between the pedicel and beak, of B10 A-line' is shorter than those of the reference varieties. The silique beak, of B10 A-line' is shorter than that of B12 A-line'. The pedicel on the silique of B10 A-line' is shorter than those of the reference varieties.
Description:
PLANT: inbred, diploid, flowers early
LEAF: wide elliptic shape, medium green, medium number of lobes, rounded to sharp type margin
FLOWER STALK: medium to strong branching
PETALS: pale yellow to yellow
SEED: ranging between small to medium and medium in size, predominantly brown
Origin & Breeding History: : 'B10 A-line' is a cytoplasmic male sterile (CMS) variety developed from a cross between the National Research Institute for Agriculture, Food and Environment (INRAE, France) brown mustard Brassica juncea Ogura CMS line and B10 B-line' in 2012. The resulting male sterile F1 plants were backcrossed 5 times to the recurrent parent, B10 B-line', from 2012 to 2015. The BC5F1 plants having the Ogura male sterile cytoplasm and nuclear genome of B10 B-line' were designated B10 A-line'. Seed increase of B10 A-line' started in 2016.
Tests & Trials: The comparative trials for 'B10 A-line' were conducted at the Ag-Quest Research Farm in Saskatoon, Saskatchewan during the 2023 and 2024 growing seasons. The plots were arranged in a RCB design with 4 replicates. Each plot consisted of 6 rows, 5 metres in length, with a row spacing of 20 centimetres. The planting density resulted in a minimum of 1800 plants per variety per year. Measured characteristics were based on 30 measurements per variety per year, except for silique characteristics, which were based on 60 measurements per variety per year. Mean differences were significant at the 5% confidence probability level based on a paired Student`s t-test.
Comparison tables for 'B10 A-line' with reference varieties 'Centennial Brown' and 'B12-A line'
Cotyledon width (mm)
| 'B10 A-line' | 'Centennial Brown' | 'B12-A line' | |
|---|---|---|---|
| mean 2023 | 18.3 | 16.2 | 16.4 |
| std. deviation 2023 | 2.35 | 1.95 | 2.27 |
| mean 2024 | 20.2 | 20.7 | 18.2 |
| std. deviation 2024 | 1.91 | 1.86 | 1.52 |
Plant height (at flowering) (cm)
| 'B10 A-line' | 'Centennial Brown' | 'B12-A line' | |
|---|---|---|---|
| mean 2023 | 80.8 | 74.2 | 71.9 |
| std. deviation 2023 | 6.68 | 8.67 | 7.53 |
| mean 2024 | 98.2 | 92.2 | 98.1 |
| std. deviation 2024 | 9.58 | 7.43 | 11.48 |
Plant total length (ground to tip of longest shoot) (cm)
| 'B10 A-line' | 'Centennial Brown' | 'B12-A line' | |
|---|---|---|---|
| mean 2023 | 122.8 | 113.4 | 110.7 |
| std. deviation 2023 | 8.53 | 8.83 | 11.29 |
| mean 2024 | 139.7 | 132.5 | 136.4 |
| std. deviation 2024 | 8.81 | 8.78 | 10.58 |
Silique length (between pedicel and beak) (mm)
| 'B10 A-line' | 'Centennial Brown' | 'B12-A line' | |
|---|---|---|---|
| mean 2023 | 33.1 | 39.6 | 34.9 |
| std. deviation 2023 | 5.35 | 3.97 | 4.28 |
| mean 2024 | 28.1 | 35.0 | 30.7 |
| std. deviation 2024 | 4.10 | 3.25 | 4.04 |
Beak length (mm)
| 'B10 A-line' | 'Centennial Brown' | 'B12-A line' | |
|---|---|---|---|
| mean 2023 | 7.7 | 8.8 | 8.7 |
| std. deviation 2023 | 1.29 | 1.31 | 1.53 |
| mean 2024 | 6.5 | 6.8 | 7.2 |
| std. deviation 2024 | 2.01 | 1.64 | 1.27 |
Pedicel length (mm)
| 'B10 A-line' | 'Centennial Brown' | 'B12-A line' | |
|---|---|---|---|
| mean 2023 | 6.8 | 8.5 | 10.5 |
| std. deviation 2023 | 1.14 | 1.33 | 3.57 |
| mean 2024 | 7.8 | 10.9 | 9.6 |
| std. deviation 2024 | 1.27 | 1.42 | 1.79 |
Click on image for larger view

Mustard: B10 A-line' (top) with reference varieties Centennial Brown' (centre) and B12 A-line' (bottom)
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